| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Pruski, Clara C (10)
|
MDRAC |
2:45.69 |
2:56.01 |
10.32 |
| 2 |
Brissett, Gabrielle S (9)
|
MDEST |
3:07.04 |
3:10.42 |
3.38 |
| 3 |
Hammer, Jacqueline J (9)
|
MDUN* |
3:17.12 |
3:16.68 |
-0.44 |
| 4 |
Perigo, Kiera M (10)
|
MDEST |
3:24.42 |
3:19.43 |
-4.99 |
| 5 |
Onyenorah, Olivia D (10)
|
MDRAC |
3:15.40 |
3:19.63 |
4.23 |
| 6 |
Belyansky, Alexandra (10)
|
MDUN* |
3:25.77 |
3:21.81 |
-3.96 |
| 7 |
Ekanayake, Nihara T (10)
|
MDEST |
3:39.63 |
3:33.64 |
-5.99 |
| 8 |
ThapaMagar, Divinee (9)
|
MDEST |
3:39.27 |
3:35.23 |
-4.04 |
| 9 |
Birdsall, Claire G (10)
|
MDEST |
3:30.89 |
3:38.97 |
8.08 |
| 10 |
Blaha, Sienna K (9)
|
MDEST |
|
4:00.02 |
- |
| 11 |
Hittle, Ava E (10)
|
MDEST |
|
4:15.95 |
- |
| 12 |
Wollenweber, Elliana S (10)
|
MDEST |
|
4:50.64 |
- |